| Data Acquisition
· Activate/deactivate acquisition on individual clamp units
· Setup and toggle between fast and slow acquisition speeds to capture transient events; speeds can be set by user and can be changed at any time during experiment
· Place time marks into file to indicate when experimental manipulations (e.g., drug additions) have been made; marks can be predefined and selected from menu or be entered from keyboard
· Graphically display current, voltage, conductance, resistance, calculated open circuit voltage and calculated Isc during data acquisition in multiple formats
Data Analysis
· Graphical/digital readout of current, voltage or conductance vs. time
· Plot raw or area corrected data
· User definable x, y scaling; zoom all, in, out, or previous; pan left, right, up or down
· Extract data under movable, adjustable width time bar in file format compatible with spreadsheet files. Extracted data may be individual points or mean values. For example, to compare measured flux to mean Isc over flux period set bar width equal to flux period, position it to start of period as indicated by Time Mark, and then press right mouse button to save to spreadsheet. Traditional method of averaging two Isc values (beginning and end values) when data are recorded manually can lead to significant errors if current is time variant. Data extraction may be specified for any of up to 8 clamps. Extracted data may be imported into commercial graphics packages to produce publication quality graphs. Standard graphs for experiment record keeping purposes may be obtained directly from analysis package.
· Linear regression analysis of conductances vs. currents can be performed on data within time bar to estimate pericellular pathway conductance. Data is saved to spreadsheet compatible file. Correct experiment design and interpretation is responsibility of user.
· Graphs (e.g., Isc vs. time, Gt vs. time) may be output to Windows compatible printers or to HP compatible (HPGL language) plotters in variety of formats. Data may be plotted without or with clamp i.d. numbers to ease reading of data when multiple traces occur on same graph. Graphs may be labeled by typing text labels on graph or by selecting text describing appropriate time marks inserted during data acquisition. Time marks may be suppressed on output or displayed either as tic marks or as vertical dashed lines. |