ACQUIRE & ANALYZE Revision II is a comprehensive data acquisition and analysis package designed specifically for use on in vitro epithelia controlled by the VCC600, VCC MC2, VCC MC6 or VCC MC8 voltage/current clamps. It consists of data acquisition hardware, interface cables and the ACQUIRE and ANALYZE (A&A II) software package. A&A II runs in the Windows® environment to measure current, voltage, conductance and resistance from one to eight tissues simultaneously. Please consult manufacturer for complete description.
Sample screen shot. Left pane: Resistance (TER) vs time. Right pane: Short-circuit current (Isc) vs. time. Graph colors, symbols, gridlines, graph parameters, tissues to plot, etc. may be set for each pane. Any number of individual panes may be defined and displayed. Other features include zoom controls, experiment protocols, event marks, data extraction tools, and quick data summary tools.
Unlike general-purpose data acquisition packages, Acquire & Analyze is written specifically for the study of epithelial tissues. For example, you want to measure the dose/response curve of an agent on the Isc and Gt by progressive additions in eight tissues. To do this you monitor the Isc and Gt on the computer until stable basal readings are attained. You place an event mark in the file and make the first addition of the agent to all 8 tissues. When the current reaches a new stable level (or a specified time has passed) you add another event mark and make the second addition of the agent.
You repeat this process until all additions have been made. To summarize the affects on Isc and Gt, use Analyze to graph the data for all 8 tissues, then display the movable data bar and click the left mouse button to position the bar over the basal currents just to the left of the first event mark. Follow this by clicking the right mouse button to save the mean values of currents, conductances and voltages to a spreadsheet. Simply repeat the mouse movements and clicks to obtain the data for each addition of the agent.
The spreadsheet, which will then contain the time of measurement, Isc, Gt and Vt for each tissue at each test concentration, can be saved and imported directly into Lotus or Excel spreadsheets for further analysis. The above hypothetical experiment would require considerable effort to set up, analyze and then integrate the results with other similar data sets using any of the general purpose data acquisition programs on the market (much less a chart recorder), whereas it is a simple, quick procedure using Acquire & Analyze.